Sorbitol- and Other Sugar-induced Expressions of the NAD+-dependent Sorbitol Dehydrogenase Gene in Japanese Pear Fruit

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Crystallization and Properties of NAD-Dependent D-Sorbitol Dehydrogenase from Gluconobacter suboxydans IFO 3257.

NAD-dependent D-sorbitol dehydrogenase (EC 1.1.1.14) was crystallized from the cytosolic fraction of Gluconobacter suboxydans IFO 3257. This is the first example of the enzyme crystallized from acetic acid bacteria. The enzyme catalyzed oxidoreduction between D-sorbitol and D-fructose in the presence of NAD or NADH. The crystalline enzyme showed a single sedimentation peak in analytical ultrace...

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Ubiquitous distribution and different subcellular localization of sorbitol dehydrogenase in fruit and leaf of apple

NAD(+)-dependent sorbitol dehydrogenase (NAD-SDH, EC 1.1.1.14), a key enzyme in sorbitol metabolism, plays an important role in regulating sink strength and determining the quality of apple fruit. Understanding the tissue and subcellular localization of NAD-SDH is helpful for understanding sorbitol metabolism in the apple. In this study, two NAD-SDH cDNA sequences were isolated from apple fruit...

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Sorbitol dehydrogenase is a cytosolic protein required for sorbitol metabolism in Arabidopsis thaliana.

Sorbitol is converted to fructose in Rosaceae species by SORBITOL DEHYDROGENASE (SDH, EC 1.1.1.14), especially in sink organs. SDH has also been found in non-Rosaceae species and here we show that the protein encoded by At5g51970 in Arabidopsis thaliana (L.) Heynh. possesses the molecular characteristics of an SDH. Using a green fluorescent protein-tagged version and anti-SDH antisera, we deter...

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Tissue-specific expression of SORBITOL DEHYDROGENASE in apple fruit during early development.

Sorbitol, the primary photosynthate and translocated carbohydrate in apple (Malusxdomestica Borkh.), is converted to fructose by sorbitol dehydrogenase (SDH; EC 1.1.1.14) which is active in apple fruit throughout development. In the apple genome, nine SDH genes have been isolated and their sequences characterized, but their individual expression patterns during apple fruit set and development h...

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Sorbitol Dehydrogenase Activity in Diabetes Mellitus and Cataract Patients

Accumulation of sorbitol in the pathogenesis of cataract in susceptible subjects has long been an issue. Sorbitol dehydrogenase (SDH) activity in 25 diabetes mellitus (DM) patients and 15 patients having cataract was investigated using 10 subjects without diabetes or cataract as the control. Results of fasting plasma glucose assay done on the DM patients showed a mean range of 64 – 144mg/dl in ...

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ژورنال

عنوان ژورنال: Journal of the American Society for Horticultural Science

سال: 2004

ISSN: 0003-1062,2327-9788

DOI: 10.21273/jashs.129.6.0870